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Scientific Proceedings (82). 



solution, and rabbit 1152 with that from 30 c.c. of the same 

 medium suspended in 2 c.c. of sterile salt solution. Cultures of 

 the bacterial suspension were made at the time of inoculation, and 

 the streptococci were found to be alive and free from contamina- 

 tion. On December 2, 1916, rabbit 1151 was again inoculated 

 with a bigger dose. These two rabbits are still in normal condi- 

 tion today — more than 4 months after inoculation. 



Of the 51 rabbits with which we have worked 11 were inocu- 

 lated with glycerinated poliomyelitis virus — 7 died and 4 are living; 

 9 were inoculated with the brain emulsions of rabbits that died 

 after inoculations with poliomyelitis virus — 2 died and 7 are living; 

 6 were inoculated with streptococci from monkeys — 3 died and 3 

 are living; 13 were inoculated with streptococci from rabbits 

 that died after inoculations with poliomyelitis virus — 5 died and 

 8 are living; 3 were inoculated with streptococci from rabbits 

 that died after inoculations with streptococci — 1 died and 2 are 

 living; 2 were syphilitic, and 7 were normal. 



Of the 18 rabbits that died after inoculations with poliomyelitis 

 virus or streptococci, none showed any of the changes usually 

 thought characteristic of poliomyelitis of man and monkey. 

 Nevertheless, we have isolated streptococci from the brains of all 

 the 1 1 on which we have made autopsies. Seven were inoculated 

 with poliomyelitis virus. 



We have also isolated streptococci from the brains of the two 

 syphilitic rabbits and 5 out of the 7 normal rabbits. Or in other 

 words, only the brains of two normal rabbits remained sterile. 



It therefore appears from our experiments that streptococci 

 are probably saprophytic microorganisms in the normal body 

 which, under conditions of lowered resistance in the course of 

 disease from other cause, acquire the power of more extensively 

 invading the tissue. They do not seem to have any etiological 

 relationship to poliomyelitis. 



We are continuing this work with more special attention to a 

 study of the tissue sections and of the cultivation of the globoid 

 bodies. 



