Anaphylaxis in the Dog. 



117 



of 10 c.c. of N hydrochloric acid.) This is then neutralized with 

 sodium hydroxide and creatinine is determined in the usual 

 manner. When only small amounts of blood are available, 1 c.c. 

 of acid is used and, after evaporation and neutralization, the 

 solution is diluted to 25 c.c. with saturated picric acid and the 

 determination is made by Folin's micro-method. The amount 

 of creatine thus found is about 4 mg. per 100 c.c. of blood. Added 

 creatine is recovered quantitatively, showing that there is no 

 conversion to creatinine in the process of coagulating the protein. 

 No claim is made that the substance reacting with picric acid 

 and sodium hydroxide under these conditions is creatinine derived 

 from the creatine of the blood. However, it is apparently not 

 formed by the condensation of glucose, urea and uric acid. A 

 solution containing 350 mg. glucose, 125 mg. urea and 5 mg. uric 

 acid was evaporated with 10 c.c. of N hydrochloric acid. There 

 was an apparent creatine content of 0.1 mg. Smaller amounts 

 of urea and glucose gave no perceptible amounts of chromogenic 

 substance. 



69 (1247) 



Anaphylaxis in the dog. 



By Richard Weil. 



[From the Department of Experimental Medicine in the Medical 

 College of Cornell University, New York.] 



1. Dogs in severe anaphylactic shock have been bled to death, 

 and the blood has been used to transfuse normal dogs. No 

 symptoms of any kind have been produced. Hence the conclusion 

 is drawn that the symptoms of anaphylaxis are not due to the 

 presence of toxic substances in the blood. 



2. The liver of sensitized dogs has been perfused with normal 

 blood by means of anastomosis of the portal vein with the carotid 

 of another dog. The blood, as it flows from the inferior cava, 

 clots within a few minutes. If the antigen (horse serum) is 

 injected into the connecting tubing, the blood in the cava soon 

 becomes less coagulable, or quite incoagulable. The conclusion 

 is drawn that the incoagulability of the blood is due, at least in 

 part, to the reaction of the sensitized liver cells to the antigen. 



