128 



Scientific Proceedings (82). 



76 (1254) 



Cellular and humoral factors in anaphylaxis and immunity. 



By W. H. Manwaring, Arthur R. Meinard and Yoshio 



Kusama. 



[From the Department of Bacteriology and Experimental Pathology, 

 Leland Stanford Jr. University.] 



Our analyses of the anaphylactic and immune reactions by 

 means of perfusion experiments with isolated rabbit and guinea 

 pig tissues have shown that the hypersensitive and immune, 

 humoral and cellular factors may coexist in the bodies of ana- 

 phylactic and immune animals in the following combinations: 



(a) Cellular anaphylaxis and apparently normal blood condition. 

 This is illustrated by the lungs of four-week anaphylactic guinea 

 pigs. Tested in the presence of normal blood, these tissues give 

 a typical anaphylactic reaction. The blood perfused through 

 normal lungs, produces no recognizable response. We refer, of 

 course, only to the immediate anaphylactic response; the slow 

 production of toxic phenomena being beyond the scope of the 

 present analyses. 



(b) Cellular anaphylaxis and humoral anaphylaxis. This is 

 illustrated by the lungs of fourteen-day anaphylactic guinea pigs. 

 Tested in the presence of normal blood, these tissues are markedly 

 hypersensitive. The blood, perfused through normal lungs, pro- 

 duces a typical anaphylactic response. 



(c) Cellular anaphylaxis and humoral immunity. This seem- 

 ingly paradoxical phenomenon is illustrated by the lungs of 

 immunized guinea pigs. Tested in the presence of normal blood, 

 these tissues are markedly hypersensitive. The blood, perfused 

 through anaphylatic lungs, prevents the anaphylactic reaction. 



(d) Cellular immunity and humoral anaphylaxis. This second 

 seeming paradox is illustrated by the hearts of anaphylactic 

 rabbits. Tested in the presence of normal blood, these tissues 

 are distinctly resistant. The blood, perfused through normal 

 hearts, produces a typical anaphylactic response. 



(e) Cellular immunity and humoral immunity. This is illus- 

 trated by the hearts of immune rabbits. These tissues are dis- 



