140 



Scientific Proceedings (83). 



fixation of complement. 1 The ice-box fixation can therefore be 

 used only as a presumptive test to eliminate the negative cases. 2 



3. As for fixation of complement at temperatures below the 

 freezing point we find that such a procedure produces undesirable 

 changes in the reagents, especially in the antigen and is therefore 

 unsuitable for the test. 



84 (1262) 



A study of the acid-base equilibrium of the blood in acute 

 bichloride intoxications. 3 



By Wm. deB. MacNider. 



[From the Laboratory of Pharmacology of the University of North 



Carolina.] 



Thirty-two dogs have been used in the initial series of experi- 

 ments. The animals were kept in metabolism cages, given 500 

 c.c. of water by stomach tube and fed on bread with a small 

 amount of meat. The urine was examined qualitatively for 

 albumin, acetone and glucose. The hydrogen ion (p. H.) con- 

 centration of the blood was determined by the method of Levy, 

 Rowntree and Marriott, and the alkali reserve of the blood 

 (R. p. H.) and the tension of alveolar-air carbon dioxide by the 

 methods of Marriott. The phenolsulphonephthalein test was 

 conducted according to the method of Rowntree and Geraghty. 



The urine during two days of observation prior to the adminis- 

 tration of the bichloride was normal. The hydrogen-ion content 

 of the blood varied between 7.4 to 7.5, the reserve alkali between 

 8.05 to 8.1, while the tension of carbon dioxide in alveolar air has 

 shown a variation between 40 to 45 mm. The total output of 

 phthalein in a two-hour period has varied from 74 to 91 per cent. 



The animals were starved for twenty-four hours prior to giving 

 the bichloride. On the days of the experiments the animals were 

 given 0.25 c.c. of a 4 per cent, solution of morphine sulphate 



1 Bronfenbrenner and Schlesinger, Proc. Soc. Exp. Biol, and Med., 1916. 

 XVI, p. 37- 



1 Bronfenbrenner and Schlesinger, Am. Jour, of Spyhilis, April, 1917. 

 ' The expense of the investigation has been aided by a grant from the Rockefeller 

 Institute for Medical Research. 



