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Scientific Proceedings (83). 



a fairly good liberation of epinephrin (at least 0.0004 mgm. 

 per minute). In the second experiment blood was drawn three 

 days after the cord section and tested on rabbit intestine and 

 uterus segments. Good concentrations of epinephrin were found 

 (1 : 1,500,000 in the fourth adrenal sample, more than 1 : 2,500,000 

 and less than 1 : 1,500,000 in the second adrenal sample). Al- 

 though the blood flow was small (0.3 gm. per minute for the second 

 sample) a substantial liberation of epinephrin was demonstrated. 



3. In one acute experiment, the spinal cord was cut between 

 the fifth and sixth cervical vertebrae. The pupil reaction was not 

 noticeably diminished. The cord was then severed between the 

 fourth and fifth dorsal vertebrae. The pupil reaction could no 

 longer be obtained. When the cord was now stimulated with 

 induction shocks between the fifth and sixth dorsal vertebrae, 

 and blood collected in the cava pocket during stimulation, good 

 eye reactions were elicited on releasing the pocket. 



4. In one survival experiment, the cord was cut between the 

 fifth and sixth dorsal vertebrae. Three days afterwards the adrenal 

 vein blood was tested by the cava pocket method but no eye 

 reactions could be obtained. The pupil gave a good reaction with 

 0.2 c.c. of 1 : 500,000 adrenalin. On intestine segments negative 

 results were obtained with adrenal blood, although a concentration 

 of 1 : 60,000,000 adrenalin in indifferent blood caused a distinct 

 effect. It was shown that the adrenal vein blood could not have 

 contained 1 : 100 000,000, and that the discharge of epinephrin 

 per minute could not have been at most 0.000003 mgm., that is, 

 not one hundredth of the output to be expected in a normal cat 

 under the experimental conditions. It was not demonstrated that 

 any epinephrin was present. 



5. In three of the cats with the cervical card transected (1 

 survival, and 2 acute experiments), the effect on the eye reactions 

 of severing nerves containing the fibers concerned in the liberation 

 of epinephrin (sympathetics and splanchnics in thorax, splanch- 

 nics in abdomen, and other nerves coming to semilunar ganglion, 

 lumbar sympathetic chain) was studied. The eye reactions still 

 obtainable from the adrenal blood after the cervical section were 

 greatly weakened or abolished after the division of those nerves. 



6. It seems to follow from these observations, that liberation 



