Effect of Coagulation of Pancreas in Situ. 151 



When, in similar experiments, enzyme concentration is varied 

 within limits suitable for such quantitative study, the rate of 

 maltose formation is found to be directly proportional to the 

 enzyme concentration, provided comparison is made at a point 

 not beyond that corresponding to a yield of about half the the- 

 oretical amount of maltose. This indicates the range within 

 which diastatic activities may be compared quantitatively. 



In the action of malt amylase upon soluble starch, we find 

 no distinct "region of linear relationship" in which the yield of 

 reducing sugar is directly proportional to time. 



Experiments with widely varied enzyme concentration show 

 that there is no cessation of hydrolysis nor true equilibrium at a 

 point corresponding to 80 per cent, of the theoretical yield of 

 maltose as claimed by some previous investigators. 



90 (1268) 



The effect of coagulation of the pancreas in situ. 



By J. Auer and I. S. Kleiner. 



[From the Department of Physiology and Pharmacology of the 

 Rockefeller Institute.] 



By injecting 10-15 c.c. of 85-95 P er cent, alcohol, usually 

 with 0.7 per cent, glacial acetic acid, into the main pancreatic 

 duct of dogs, we coagulated at least 95 per cent, of this organ in 

 successful experiments. The extent of this coagulation was deter- 

 mined by a careful inspection at the time of injection, by re- 

 operation after a number of weeks, and by autopsy and micro- 

 scopical examination. The external secretion of the gland was 

 abolished in all experiments. 



Our material is formed by 19 dogs, of which six lived four weeks 

 and longer; one of the four dogs still living is in excellent condition 

 104 days after the operation. The blood and urine were examined 

 at frequent intervals, daily when necessary. The dogs were fed 

 a regular mixed diet composed of about 100 grams of cooked 

 meat scraps, 4-500 grams of bread-meat broth mush, 50 grams 

 ground bone and occasionally 10-60 grams of lard. Water was 

 given freely. 



