Immunization against Cyanolophia. 159 



inoculated with brain tissue which had been during shorter or 

 longer periods in a plasma medium. Table III gives the exact 



table in. 



0. 



X. 



No. 47. 



1 1 



1 1 



1 1 





1 Z ULCU 



56 died 



!3 



UlcU 



0 / 





OA 



C8 



j° 





1 c 

 *3 







26 died 



60 





27 died 



6l 





28** 



62 





29 



63 





30 



64 





31 



65 





32 







33 







34 







35 died 







36 







37 died 







38 







50 





numbers and shows how often these chickens had been inoculated 

 with virulent brain in a plasma medium. 



A few chickens were treated with virulent sera which had been 

 kept on ice (Table IV). 



TABLE IV. 



Serum O. 



Serum T. 



Serum Q. 



Serum R. 



Serum Z. 



24 



24 



24 



52 died 



67 



25 



25 



25 





73 



28 



28 







74 



From the chickens prepared in this prescribed manner, I 

 chose from the different groups several animals. For example: 

 No. 15 and No. 77 had received only desiccated brain, No. 15 

 nine times and No. 77 six times. Both died, together with control 

 animal No. Ui, after being inoculated with virulent brain No. Q. 

 No. 11 and No. 68 which had been treated with desiccated brain 

 and with brain which had been attenuated in a plasma medium, 

 died, together with control animal Si, after application of virulent 

 brain No. Q. That proves that continued doses of desiccated brain 

 and of brain attenuated in a plasma medium do not protect the 

 chicken against Cyanolophia. 



