164 



Scientific Proceedings (84). 



sister cells being isolated every day. The results may be sum- 

 marized in six groups including 5 consecutive days each, as follows: 





Controls. 



Vitamine-treated. 



Divisions. 



Rate. 



Divisions. 



Rate. 





45 



I.80 



42 



1.68 





28 



1. 12 



26 



1.04 





16 



O.64 



24 



0.96 





30 



1.20 



25 



1. 00 





19 



O.76 



24 



O.96 



Group F. Sixth 5 days 



15 



0.00 



19 



0.76 



Totals 



153 



1.02 



160 



1.06 



4. The final experiment was introduced to see if the vitamine 

 treatment will stimulate subsequent metabolic activities. Indi- 

 viduals that had been treated for twenty-four hours were isolated 

 in the usual medium without vitamine. Here again 5 lines with 

 controls were carried on for 15 days. The control animals 

 divided 64 times, the post-vitamine animals 63 times with division 

 rates of 0.85 and 0.84 respectively. The minute differences in all 

 these results fall within the limits of experimental error and show 

 that the vitamines as used have no effect on the nutrition of 

 Paramecium cells. 



95 (1273) 



Further observations on pancreatic vitamine. 



By Walter H. Eddy. 



[From the Department of Pathology, New York Hospital, New York 



City.] 



In connection with the use of pancreatic vitamine two problems 

 have arisen which must be solved if the treatment is to be more 

 than empirical. One is the establishment of a satisfactory method 

 of dosage determination and the other is the explanation of the 

 method of action of the vitamine upon the organism. In the 

 previous paper is reported an attempt to solve the latter problem. 

 In this paper I wish to present certain collated observations bearing 

 upon the dosage problem. 



