Further Observations on Pancreatic Vitamine. 165 



I. A Comparsion of Pancreatic Vitamine and Yeast Vitamine. 



In a recent paper Seidell 1 has outlined the result of his studies 

 of autolyzed yeast filtrate. Making use of similar methods and 

 using an arbitrary standard of 2.7 gm. pancreas to each cubic 

 cm. of water extract as my solution I obtain the following results: 



table. 





Autolyzed Yeast 

 Filtrate(Seidell). 



Pancreatic 

 Prep. I. 



Extract 

 Prep. II. 



1. Nitrogen in 100 c.c 



2. 5 gm. Lloyd reagent shaken with 100 



c.c. extract cont 



3. S gm. Lloyd reagent shaken with 



filtrate from 2. cont 



2.0 gm. 

 0.09 gm. N. 

 0.073 gm- N. 



0.754 gm. 

 0.150 gm. N. 

 0.088 gm. N. 



0.644 gm. 

 0.159 gm. N. 

 0.102 gm. N. 



From experiments conducted by Williams and Seidell 2 they con- 

 clude that vitamine probably contains approximately 50 per cent, 

 nitrogen. Other experiments indicate that practically all the 

 vitamine is removed in the first shaking with the Lloyd reagent. 

 My own experiments confirm their view on the latter point. 

 In regard to the first conclusion I have no data. Accepting their 

 assumptions in both particulars it follows that: 



Yeast Filtrate. Pancreatic Ext. 



100 c.c. of extract contains 0.18 gm. Vitamine 0.30 gm. Vitamine. 



Both experiments show that the Lloyd reagent adsorbs nitrogenous 

 substances that are not vitamines. Furthermore it is reasonable 

 to suppose that some of the N of the first shaking is not vitamine N. 

 Hence the vitamine content given above must be taken not as the 

 absolute figure but as the maximum content possible. 



Seidell has also found that 1 c.c. of yeast filtrate given every 

 other day protects pigeons from polyneuritis, and that these birds 

 consume 30 gm. of food per day. One cubic cm. of yeast filtrate 

 would therefore contain not more than 0.0018 gm. of vitamine, 

 hence he concludes that 0.0018 gm. vitamine to each 60 gm. of food 

 is a proper vitamine ratio for a pigeon, i. e., one containing 0.003 

 per cent, vitamine. In experiments with rats and babies my 

 aim has been growth rather than antineuritic tests. In my experi- 



1 Atherton Seidell, "The Vitamine Content of Brewer's Yeast," Journ. Biol. 

 Chem., XXIX, 145. 



2 Williams, R. R., and Seidell, A., /. Biol. Chem., XXVI, 431- 



