Lipoid-free Immune Serum. 



Lloyd reagent both give the reaction and there is a marked lessen- 

 ing in color intensity when the extract is made of the second 

 shaking of Lloyd reagent. 



6. That the color is due in part to the vitamine is shown by 

 the fact that a portion of the extract carried through the com- 

 plete Funk preparation process gives the color strongly. 



7. The color can be obtained with the aid of the centrifuge 

 in a clear form usable against a uric acid standard. 



These results show that the substance adsorbed by the Lloyd 

 has the power to give the color reaction and indicates that there 

 is also present some substance other than vitamine which has 

 this power. Granting this fact, however, the results offer a 

 method of standardizing dosage that seems to give promise of 

 practical value. 



96 (1274) 



Lipoid-free immune serum does not produce passive immunity 

 to transplanted tumors. 



By G. L. Rohdenburg, M.D. (by invitation). 



[From Columbia University, George Crocker Special Research Fund, 

 F. C. Wood, Director.] 



Although it has been repeatedly attempted, no one has suc- 

 ceeded in transferring immunity to transplantable tumor in 

 animals by injecting the serum of immune animals. Our experi- 

 ments on passive immunity, duplicating in part the work of others, 

 have also led to negative results, and are recorded simply to add to 

 the existing data. 



Serum was obtained from normal rats, from rats immune 

 to inoculations of the Flexner-Jobling rat carcinoma, and from 

 guinea pigs sensitized by three injections of the Flexner-Jobling 

 tumor in doses of 1 gm. These sera were injected daily in doses 

 of 1 c.c. for nine days previous to an inoculation of 0.003 g m - of 

 the Flexner-Jobling tumor, and a control series of non-injected 

 animals was inoculated at the same time, twenty-five animals being 

 used for each series. Four weeks after inoculation, no immunity 

 was demonstrable in any of the treated groups, which showed 

 from 88 to 92 per cent, takes, as compared with 92 per cent, 

 takes in the control groups. 



