170 



Scientific Proceedings (84). 



98 (1276) 



The non-protein nitrogenous constituents of normal human 



muscle. 



By Alma Hiller, Samuel W. Clausen and Herman O. 



MOSENTHAL. 



[From the Johns Hopkins Hospital, Baltimore, Md.] 



The material for these analyses was obtained at operations for 

 severe lacerations, carcinoma of the breast, or gangrene of the 

 extremities. Only such specimens were utilized as were not in- 

 volved by the pathological process. This seemed to be the only 

 available source from which muscle tissue that might be regarded 

 as normal could be procured. In every case the blood of these 

 subjects was analyzed for its non-protein nitrogen as well as urea 

 content. Any patients in whom these were above the normal 

 would not have been utilized in this series. As a matter of fact, 

 no abnormal bloods were encountered among these cases. 



The maximal, minimal and average figures, as well as the 

 number of determinations for each substance, are given in the 

 appended table. 



The Non-protein Nitrogenous Constituents of Normal Human Muscle. 



Substance. 



No. of 

 Deter- 

 mina- 

 tions. 



Mgm. 



Maxi- 

 mal. 



. per 100 

 Muscle. 



Mini- 

 mal. 



Gms. 



Aver- 

 age. 



Remarks on Methods. 



Non-protein nitrogen. . 



19 



234 



100 



166 



Alcoholic extract. 



Non-protein nitrogen. . 



7 



346 



26S 



292 



Extraction with heat and 













acetic acid, evaporation to 













small volume and extrac- 













tion with trichloracetic 













acid. 





19 



25 



8 



13 



Alcoholic extract. 





12 



404 



212 



350 



Folin's method. 



Kreatinin 



13 



12 



2 



5 



Folin's method. 



Amino acid nitrogen . . . 



12 



42 



16 



32 



Van Slyke's method. 



The results for the non-protein nitrogen are much lower than 

 they should be. These data were obtained by extracting the 

 muscle tissue by alcohol. Alcohol does not dissolve the kreatin. 

 Since this substance forms such a large portion of the non-protein 



