172 



Scientific Proceedings (84). 



sion is justified that as far as reaction is concerned, the concentra- 

 tion of hydrogen ions is adequate for autolysis. 



Can protein hydrolysis be demonstrated coordinate with the C H ? 

 In the experiments described above, the variation of C NH2 nitrogen 

 was found to be as follows: 



The conclusion is here justified that there is evidence of 

 hydrolysis of the proteins correlative with the development of 

 acidity. 



In work with inorganic colloids and catalysis, it has been 

 shown that there is a relation between colloidal dispersion and 

 catalysis. It is known that brain tissue hydrolyzes very much 

 more slowly in vitro than in vivo and than other tissues. The 

 question arose as to the following point: 



Can the autolysis of slowly -autoly zing tissues such as the brain, 

 be accelerated by modifying the dispersion of the colloids? Dog's 

 brain was extracted in the cold with ethyl ether and the residue 

 tested against control tissue from the same brain : 



Initial. 7 Days. 



Control . 0.55 rngm. 1.25 mgm. NH 2 nitrogen (Van Slyke gasometric method). 

 Ether. . .0.67 mgm. 0.65 mgm. NH 2 nitrogen (Van Slyke gasometric method). 



No acceleration (but rather an inhibition) of autolysis occurred. 

 In the use of CHCI3, a similar result was obtained. Again, by 

 saturating the unsaturated compounds of serum, which inhibits 

 autolysis to be some degree, no difference between control and 

 experiment was obtained; the compounds were hydrogenated 

 and iodized by distributing the serum upon the walls of a large 

 separatory funnel. 



Time. 



Mgm. 

 Per Cent. 



Control, vein 



Control, mesenteric vein . . 

 After 45 minutes ligation . 

 After 240 minutes ligation 



12. 1 



7-7 

 7-7 

 8.8 



