Survival of Engrafted Ovarian Tissue. 43 



26 (436) 



The survival and growth of subcutaneously engrafted ovarian 

 and testicular tissue. 



By C. C. GUTHRIE. 



\From the Physiological Laboratories of Washington and Pittsburgh 



Universities^ 



Fragments of ovaries and testicles in chickens, and testicles in 

 guinea fowls were removed and placed beneath the skin. Other 

 similar fragments were placed in the peritoneal cavity. After 

 several months the engrafted tissues were examined and compared 

 with each other and also with the tissue left in normal situation. 



Results. — When engrafted in favorable situations, i. e., in close 

 proximity to large blood vessels, 1 both subcutaneous and intraperi- 

 toneal ovarian tissues make a good growth in chickens, being 

 very similar both in gross and microscopical appearance to normal 

 ovarian tissue. In agreement with Lode, 2 testicular tissue in 

 chickens was found to give good results being very similar to the 

 results for ovaries. The same was also found to be true for guinea 

 fowls. Such engrafted tissues contained numerous spermatozoa. 



Results of exchanges of such tissues between the sexes as 

 well as between different species have thus far been negative, but 

 it would be premature to draw definite conclusions from the results. 



Using juice prepared from engrafted as well as from normal 

 testicular tissue investigations are being made to determine the 

 feasibility of thus artificially fertilizing hens with the view of testing 

 for a "soma" or "foster father" influence on the sperm; and 

 also to obtain more data on the role of the accessory sexual 

 secretions. 



1 Knauer, Cent, fur Gynakol., 1896, xx, 524 ; Arch, filr GynakoL, 1900, lx, 322. 

 'Lode, Wiener klin. Woch., 1895, viii, 345. 



