4 8 



Scientific Proceedings (36). 



be removed; 75 c.c. of urine plus 2.5 c.c. concentrated sulphuric 

 acid are heated in an autoclave, for one and one half hours at 175 0 . 

 The urea is entirely changed to ammonia, which is boiled off after 

 adding 10 gm. of calcium oxide and a piece of paraffin. The 

 ammonia-free filtrate is concentrated to a small volume, diluted to 

 25 c.c, and amino nitrogen determinations made on 10 c.c. portions. 

 In blank determinations, 11.55 m g- nitrogen as alanine were 

 added to a normal urine. The increase in amino nitrogen was 

 1 1.47 and 11.58 mg. n duplicates. Determinations on a num- 

 ber of normal urines indicate a normal amino nitrogen of 2.0 per 

 cent. ±0.5 per cent, for a normal urine. The study of pathologi- 

 cal urines is being undertaken, as the amino determination may be 

 of value in indicating conditions where physiological oxidation of 

 protein nitrogen is incomplete. 



30 (440) 



Note on the production of glycosuria by parathyroids, 

 pancreas and the infundibular extract of the 

 pituitary. 



By ISAAC OTT, M.D., and JOHN C. SCOTT, M.D. 



In experiments upon cats we have found that injection per 

 jugular of filtered watery solution of the parathyroid or the 

 pancreas produced 3 to 4 per cent, of sugar in the urine. Injec- 

 tions into abdominal cavity of the pancreas also produced glyco- 

 suria. Borchardt has shown that the pituitary gland as a whole 

 causes sugar to appear in the urine. We have found 1 c.c. of the 

 20 per cent, extract of the infundibular part (Burroughs, Well- 

 come and Co.) of the pituitary by the jugular causes about 3 per 

 cent, of sugar to appear in the urine. We took care that the 

 binding down and etherization did not cause any sugar to be 

 present in the urine of our animals. Falta and Priestly did not find 

 any increase of the sugar in the blood of the rabbit by relatively 

 large doses of infundibulin. The presence of glucose was always 

 determined by Fehling's, fermentation, and phenylhydrazine tests. 



