7 6 



Scientific Proceedings (36). 



2. All vaccinating properties of the active egg-white residue 

 appears to be completely absent. This, also, would be expected 

 from the prolonged heating to which it has been subjected, which 

 is in complete accord with the effect of heat on whole protein. 



The toxic and vaccinating properties are first lost and, later, 

 the sensitizing properties. The finding of the residues incapable of 

 sensitizing completes the parallel of the effect of heat on unbroken 

 proteins. 



If the vaccinating properties of the typhoid residue are lost, as 

 might be inferred from the loss of those of the egg-white, the use 

 of large doses of the typhoid residue to absorb the bacteriolytic 

 ferment in typhoid fever showing severe toxic symptoms, as sug- 

 gested by Vaughan, would probably not be successful. Experi- 

 ments now being carried out on the typhoid residue, will, we hope, 

 soon permit us to make a definite statement on this point. 



3. Besredka's Antianaphylactic Vaccination. — Following the 

 method of Besredka, precipitating serum protein with alcohol and 

 extracting the precipitate with physiological salt solution, a filtrate 

 was obtained, called by him " petit serum." According to his 

 article this "petit serum" was never toxic to a serum sensitized 

 guinea pig, and yet it vaccinated, but did not sensitize. Proceed- 

 ing on the theory that Besredka's results were due to the dilution 

 of this protein, not to a separation of the substances, we concen- 

 trated the filtrate of his "petit serum." On intracardiac injection 

 five out of seven serum sensitized guinea pigs gave typical ana- 

 phylactic symptoms, followed by death. This proves that the lack 

 of toxicity in his work had been due to dilution and not to freedom 

 from the substance producing toxic substances. 



The sensitizing action was tested out, and in certain doses we 

 were able to sensitize animals, not as regularly as with whole 

 serum, but sufficiently to show the presence of sensitizing proper- 

 ties in the "petit serum." 



4. Absence of Antianaphylaxis in Rabbits — In conclusion we 

 wish to confirm the results of Arthus, Lesue, and Dreyfus, that 

 antianaphylaxis is not produced in the rabbit by the methods that 

 ordinarily so readily and rapidly produce it in the guinea pig. 



The present attitude of comparing man's sensibility to serum 

 with that of the guinea pig is possibly an error. Man, perhaps, 



