io6 



Scientific Proceedings (38). 



2. 3 c.c. of this unconcentrated filtrate gave a marked reaction. 



3. 10 drops of the crude tuberculin used on March n gave a marked reaction, 

 but 10 drops of crude tuberculin used on March 9 were inactive. 



4. I drop of the tuberculin of January gave a very marked reaction. 



5- 5 c.c. of this unconcentrated filtrate finally gave a marked reaction. 

 Protocol of March 18. — Dog, mongrel collie, 75 lbs., sensitized March 17, with 

 5 c.c. of the unconcentrated filtrate used March 16. 



1. 10 drops of crude tuberculin used March 11 gave no reaction. 



2. 5 c.c. unconcentrated tuberculin used to sensitize the dog failed to react. 



3. \y 2 drops of crude tuberculin of January gave a marked reaction. 



Protocol of March 23. — Bulldog, 25 lbs., sensitized March 22, with 5 c.c. of the 

 unconcentrated filtrate. 



In this series of injections the unconcentrated filtrate was heated at different 

 temperatures for one hour and water added afterwards to make the volume equal to 

 the original volume. 



The following temperatures were used : 



35° C. original incubator temperature. 

 50° C. — 2 hours. 

 6o° C. — 1 " 

 70 0 C. — 1 " 

 8o° C. — 1 " 

 ioo° C. — 1 " 

 105 0 C. — 1 " 



These heated preparations were made by Mr. Banzhaf ; 5 c.c. were given at each 

 injection. The injections were begun with the preparations which had been heated to 

 105 0 C. and given in order down to the original incubator filtrate. The 105 0 C. heated 

 preparation gave only an indication of a reaction. The other preparations down to the 

 original gave marked reactions. 



The original unconcentrated filtrate gave a reaction which was not so marked as 

 the heated preparations, but a later injection gave just as good a reaction. This dog 

 which received these injections was used to test the sera of three calves which had been 

 given intravenous injections of virulent cultures of B. tuberculosis, bovine. 



These calves were killed and autopsied with results as follows : Calf No. 24. 

 Strain 377. Injected Oct. 6, '09. Killed Mar. 22, '10. Small % inch nodule with 

 caseous center at site of injection. 



Calf No. 17. Strain 96. Injected Aug. 12, '09. Killed Mar. 22, ' 10. % inch, 

 tubercle in prepectoral node. 



Calf, No. 25. Strain 558. Injected June 10, '09. Killed Mar. 22, '10. 

 Fibrous area at site of injection, small amount of caseous material. Calcification. 



These calves gave practically no physical signs of tuberculosis. The injection of 

 4 c.c. of serum of each of these calves produced the characteristic reaction which we 

 have described. Serum drawn from a normal calf did not give a reaction. This calf 

 was kept in the same stable and under the same conditions as the three injected calves. 



Protocol of March 25. — Bull dog, 35 lbs., sensitized with 10 c.c. of the uncon- 

 centrated filtrate. Primarily used to test a normal calf kept with and under the same 

 conditions as the three previous calves. 



1. 3 and 4 c.c. respectively of this normal calf serum failed to give a reaction. 



2. Injections of 4 c.c. from each of the tubercular calves failed to react. 



3. 5 c.c. of unconcentrated filtrate, which had been heated to 6o° C. and tested on 

 March 23, gave a marked reaction. 



