Isolating the Cerebro-medullary Circulation. 113 



neutral sulphur, wherein deficient oxidation would be most apt to be 

 manifested, with the amounts of a normal child, shows very slightly 

 higher figures but the difference is not sufficient to justify the conclu- 

 sion of deficient oxidation. 



71 (481) 



On parenteral protein assimilation. 



By P. A. LEVENE and G. M. MEYER. 



\From the Department of Chemistry of the Laboratories of the Rocke- 

 feller Institute for Medical Research?^ 



The results of recent work on the fate of protein introduced 

 parenterally led to the conviction that such protein is assimilated 

 and utilized by the organism in the same manner and in accordance 

 with the same laws as protein ingested per os. However, there 

 exists a considerable divergence in the views on the mechanism by 

 which this assimilation is accomplished. 



Very recently Freund advanced a theory that protein intro- 

 duced into the organism subcutaneously or through the circulation 

 is eliminated into the intestinal tract where it undergoes the usual 

 digestion and absorption. 



The present investigation aimed to test the correctness of the 

 last theory. An animal deprived of its jejunum and ileum was 

 placed on a standard diet and brought into a condition of nitro- 

 genous equilibrium. On the days of experiments the animal 

 received a subcutaneous injection of horse serum, heated for one 

 half hour at 60 0 C. The volume of injected serum was equivalent 

 to 1.5 grams of nitrogen. The elimination of additional nitrogen 

 was followed for several days following the days of the serum 

 injection. In all experiments was noted a complete retention of the 

 protein introduced parenterally in the same manner as this occurs in 

 normal animals. Thus the theory of Freund is contradicted by the 

 results of our experiments. 



72 (482) 



A method of isolating the cerebro-medullary circulation. 

 By ARTHUR BRADLEY ElSENBREY. 



[From the Carnegie Laboratory of New York University. ] 

 In a recent study by Dr. R. M. Pearce and myself of the 

 mechanism of certain experimental conditions of low blood pres- 

 sure, the following difficulties were encountered : 



