124 



Scientific Proceedings (38). 



injection of 1 c.c. of a 10 per cent, suspension of washed goat's 

 corpuscles per kilo body weight. The control animal received 

 intraperitoneally an emulsion of spleen from a normal dog (see 

 curve). 



Never does the serum of the control dog cause agglutination 

 in greater dilution than 1:48. The agglutinating power is, on the 

 whole, less than 1:24. The serum of the dog which received the 

 immunized dog's spleen showed an increase in agglutinin from the 

 start. Rising from a normal of 1:24 the serum agglutinates on 

 the fifth and seventh day in a dilution of 1:384. From the eighth 

 to the thirteenth day the serum agglutinates in a dilution of 1:96 ; 

 and on the seventeenth day, the day of testing the fluids, the serum 

 possesses an agglutinating value of only 1:48. 



A future paper will give in greater detail this and other experi- 

 ments which have yielded similar results with respect to antigen- 

 fixation by the spleen. The mechanism of subsequent antibody 

 formation in the recipient, whether by growth of the living cells 

 or a second fixation of antigen liberated by the dead or dying 

 splenic cells remains an open question. The results so far obtained 

 warrant the conclusion that the spleen fixes antigen ; and that this 

 organ is, therefore, necessarily concerned with antibody formation. 



The same method has been used with lymph glands and bone 

 marrow with negative results. The work is being continued along 

 this line with the hope of proving the point directly and conclusively 

 by the successful transplantation of the spleen. 



77 (487) 



The concentration of ammonia in the blood of dogs and 

 cats necessary to produce ammonia tetany. 



By CLARA JACOBSON. (By invitation.) 



\From the Hull Physiological Laboratory of the University of 



Chicago.] 



The importance of this subject rests in its relation to the 

 markedly increased ammonia content of the blood of animals in 

 parathyroid tetany. In a recent paper, 1 evidence was presented 

 which indicates that this increased ammonia is apparently asso- 



1 Carlson and Jacobson : American Jour, of Physiol., 1910, xxv, 403. 



