Scientific Proceedings. 



15 



2. The influence of nuclein, nucleic acid and of adenin on the 

 uric acid elimination was studied. It was observed that all these 

 substances caused an increase in the uric acid elimination. 



3. The fate of thymin ingested with the food was investigated. 

 The greater part of the ingested thymin was recovered from the urine. 



4. An attempt was made to find thymin in the urine of the 

 same dog after feeding on nuclein and on nucleic acid. The en- 

 deavor was not successful. 



5. The influence of a diet containing a small proportion of pro- 

 tein but abundant in calories was studied. It was noticed that this 

 diet occasioned an increase in the uric acid output. 



6. The influence of fasting on the uric acid output was ob- 

 served. It was noted in the course of the fast that the uric acid 

 elimination was above the normal. 



15 (158) 



On the fractionation of agglutinins and antitoxin. 

 By R. B. GIBSON and K. R. COLLINS. 



[From the Research Laboratory of the Department of Health, of 

 New York City.'] 

 E. P. Pick in 190 1 associated a number of anti-substances indi- 

 vidually with the one or the other of the two serum globulin frac- 

 tions of the Hofmeister classification. In the pseudoglobulin [3.4 to 

 4.6 sat. (NH 4 ) 2 S0 4 solution 1 ] group of antibodies he placed the 

 diphtheria and tetanus antitoxins and the typhoid agglutinin of horse 

 serum ; the lower or euglobulin fraction (2.9 to 3.4 sat.) com- 

 prises diphtheria and tetanus antitoxin and cholera lysin in the 

 goat, typhoid agglutinin in the goat, rabbit and guinea pig, and finally 

 cholera agglutinin in the horse and goat. It becomes possible, 

 according to Pick, to separate the individual specifically reacting 

 anti-substances by fractioning appropriate mixtures of sera. Such 

 a possibility suggested the application of this method to the further 

 study of certain anti -bodies, especially of the relation of specific and 

 group agglutinins developed by immunization against a single strain 

 of organism. Preliminary experiments in the course of our inves- 



1 The degrees of saturation, as here expressed, indicate a concentration equivalent to 

 a content in 10 c.c. of solution of 3.4 c.c. and 4.6 c.c. of saturated ammonium sulphate 

 solution respectively. 



