Scientific Proceedings. 



65 



In every instance the donee experienced a heightened vitality, and 

 in the absence of serious organic disease the patient became buoy- 

 ant, even jocose. Some had chills during transfusion or soon after, 

 and a majority showed some febrile reaction later. 



47 (190) 



A preliminary report on the direct transfusion of blood in 

 animals given excessive doses of diphtheria toxins. 



By GEORGE W. CRILE and D. H. DOLLEY. 



\From the Laboratory of Surgical Physiology, Western Reserve 

 University Medical College^ 



Technique. — The dog was given subctitaneously the dose noted. 

 After waiting a certain time an anastomosis was made between one 

 of his vessels (usually, for convenience, the external jugular) and an 

 artery (carotid) of a donor, of equal or usually larger size. When 

 this was perfect, the toxic dog was bled, usually from a femoral 

 artery, as rapidly as possible, to complete exsanguination, and the 

 transfusion was in no case started till cessation of respiration gave 

 warning of the limit's being reached. When this occurred the 

 blood was allowed to flow, under control, until the pulse returned 

 in every case to a better quantity than before. The time taken in 

 transfusing was usually about 15 minutes. (The venous anas- 

 tomosis was made because more blood went into the donee by it.) 



Weight of the donee. 1 Dose. 4 Time of bleeding after dosage. Result. 3 



kg. c.c. hrs. 



3.13 0.025 2 4 Died in 84 hours. 



4.8 0.025 20 Died in 1 20 hours. 



2.8 0.015 l l% Died in 120 hours. 



7.3 0.015 3 Died in 84 hours. 



4.5 0.015 *}4 Died in 10 days. 



1 Not essential as the donee bled completely and the transfused amount could 

 only be estimated. 



2 The toxin used was a fresh supply (1906). It was not so definite in its effect as 

 regards time as the first. Four control dogs, with 0.015 c - c - each, died in 3, 5, 7 and 8 

 days respectively ; one with 0.02 c.c. died in 3 days and one with 0.025 c.c. , in 2 days. 



3 Autopsies were performed on all these dogs, in which the findings were the same 

 as in the controls, i. <r., varying degrees of hemorrhagic enteritis, focal hemorrhages in 

 the kidneys and marked cloudy swelling of the liver and kidney, with jaundice. In 

 some, focal necroses of liver and kidney were apparently present. The microscopic 

 part has not yet been worked up. 



