66 Society for Experimental Biology and Medicine. 



The experiments were next varied in this way ; instead of 

 treating a toxic dog with normal blood, an exsanguinated normal 

 dog was transfused from one which had been given the toxin sub- 

 cutaneously some time previous, as noted below. To be sure of 

 an excess of toxin the dose was doubled. The technique was the 

 same, under ether with careful asepsis. The vascular anastomosis 

 was made before the normal dog was bled. The time is calculated 

 from when the dose was given till the transfusion was started. In 

 the fourth experiment one donor (St. Bernard dog weighing 40 k.) 

 supplied three small dogs one after another with sufficient blood. 



Summary of Effects on Donees. 



Elapsed time between dosage Dose given donor. 



Result. 



and transfusion. 

 6 hours 



4 hours 

 3 hours, 50 minutes 

 'A. l hour, 5 minutes 



B. 3 hours, 10 minutes 

 L C 5 hours, 10 minutes 



c.c. 



0.015 



0.030 

 0.030 



0.025 

 0.025 



0.025 



Lived 3 weeks under observation ; 



entirely healthy. 

 Same. 

 Same. 



Developed paralysis of both hind legs 

 in 10 days. 



Died in 15 days. Widespread bron- 

 cho-pneumonia (accidental infec- 

 tion, no other lesion). 



No ill effects. 



No further observations of these dogs were made. The para- 

 lyzed dog is certainly suggestive of diphtheritic paralysis. The dog 

 lived for over a month in this paralyzed state, but when he died I 

 was not informed and the body was buried. 



Intravenous Inoculation. 



Elapsed time between dosage Dose given donor. Result, 

 and transfusion. c.c. 



24 minutes 0.03 Died in 6 days (usual postmortem 



appearance). 



I. Summary of the Experiments on the Treatment of Diphtheric Toxemia 



by Bleeding Alone. 



Dose per kg. 



Elapsed time between 



Approximate amount of 



Result. 



c.c. 1 



dosage and bleeding. 



blood removed. 



(In hours after dosage.) 





hrs. 





O.OI5 



18 





Died in 36 hours. 



O.OI5 



19 



44 within 60 " 



O.OI5 



7 





« 45 « 



O.OI5 



7 





u 45 « 



O.OIO 



3 





« « 48 " 



O.OIO 



3 







'0.015 c - c - °f the toxin per kg. killed control dogs in two days, while o.ol c.c. 

 averaged somewhat over three days in causing death. 



