82 Society for Experimental Biology and Medicine. 



to the amount of 200 cc. three times a day, but absolutely no food. 

 At the conclusion of the fast his weight was one hundred and 

 twenty-five pounds, a loss of fourteen pounds. 



Unfortunately the fast had already begun when the other of 

 us (R) was asked to make opsonic investigations. The first 

 observation was then made on December 28th, thirty-six hours 

 after the fast had started ; the blood was taken at 8 : 30 p. m. 

 From this date onwards observations were made daily, except on 

 January 1st and January 3rd. The blood was drawn at the same 

 hour each day (10 a. m.) ; on the last day, however, the blood 

 was examined at 4 p. m., shortly before the conclusion of the 

 fast. 



After the fast the blood was examined five times over a period 

 of two weeks. The blood was taken in the afternoon. An 

 eighteen-hour culture of staphylococcus upon plain agar was used 

 for the preparation of the emulsion. This organism was used on 

 account of the fact that with it accurate results can be obtained 

 and also on account of the fact that we were somewhat unprepared 

 for the work which we took up hurriedly at a few hours notice. 

 The control serum was that of an individual whose opsonic power 

 had been shown (by pool) to be normal to this organism. The 

 technique employed was that of Wright and Douglas. 



The accompanying diagram gives a clear picture of the result 

 obtained. 



DEC.06. JAN 07 



27 28 29 30 31 \ 2 3^-5 6 7 B 9 10 II 12 13 14 15 16 17 18 19 20 



OPSONIC 

 INDEX 

 \ 2. 



09 



0 B 



0 7 



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B 



















V- 



< 























































































5 



















o 

 z 



































i 



















Mi 



































