Scientific Proceedings. 



87 



inoculations. Enormous numbers of spirochete, apparently iden- 

 tical with those which occur in the tumors of the mouse, were 

 found in the myocardium and in the lung. 



Another mouse, twice inoculated with the Ehrlich "Stamme 11" 

 tumor, died three months after the second inoculation. Neither of 

 the inoculations was followed by any growth of the tumor. There 

 was a long-standing inflammation of one foot and leg. Spirochetal 

 were found in small numbers about the inflammation in the foot, 

 and in enormous numbers in the mediastinum about the bronchi 

 and large veins. The tubules of the kidney also contain large 

 numbers, but they here appear to be undergoing disintegration and 

 and are not so readily distinguished. The organisms in these two 

 cases are of the form of a relatively thick, broad spiral, and have 

 at one end a flagellum, which is less intensely colored than the 

 body of the organism. 



The spirochetal found in these two cases appear to be identical 

 with those found in the tumors of the mouse. 



58 (201) 



Concerning the neutrality of protoplasm. 



By LAWRENCE J. HENDERSON (by invitation). 



[From the Laboratory of Biological Chemistry of the Harvard 



Medical School.'] 



It is desirable, both on account of the normal production of acid 

 during metabolism, and because of the production of acid under 

 pathological circumstances, to study the adjustment of equilibrium 

 in protoplasm whereby neutrality is maintained. In undertaking 

 this study the equilibrium in mixtures of sodium hydrate, phos- 

 phoric acid and carbonic acid has been studied. 



As a result of the investigations it appears that in the presence 

 of both free and combined carbonic acid in measurable amount, 

 such mixtures are precisely neutral to rosolic acid, and that the 

 amount of sodium bicarbonate in such mixtures can vary greatly 

 without great variation in the ratio between mono-sodium phos- 

 phate and di-sodium phosphate. These results are in accord with 

 the theory, based upon the ionization constant of carbonic acid 



