Scientific Proceedings. 



129 



His work has been repeated in this laboratory as nearly as was 

 possible from the meagre description given of his technic. Three 

 old and three young rabbits were injected under the skin of the 

 ear with the Scharlach R-olive oil solution, and the injected 

 tissue excised and examined at times varying from seven to sixty- 

 one days. Our results show that the solution has absolutely no 

 influence on the epithelial elements, but acts as a mild irritant, 

 inducing a chronic inflammation with slight reaction on the part of 

 the connective tissue in the case of the old rabbits, and a greater 

 reaction with the formation of foreign body giant cells in the case 

 of the young rabbits, the conclusion being that the attraxin 

 theory is without sound foundation, in so far as " Scarlet-oil " is 

 concerned. 



91 (234) 



The effects of struggle on the content of white cells in the 



lymph. 



By F. PEYTON ROUS. (Communicated by ALDRED 

 S. WARTHIN.) 



[From the Pathological Laboratory, University of Michigan.'] 



As the first stage of an investigation into the content of white 

 cells in the lymph under certain common physiological and patho- 

 logical conditions, the author has studied the effects on this con- 

 tent of variations in muscular activity. The lymph running from 

 the thoracic duct was collected in a special mixing-tube — 3 c.c. 

 of lymph to 3 c.c. of a 4 per cent, solution of sodium citrate in 

 0.8 per cent, salt solution — tinting accomplished with a trace of 

 methyl violet, and counts made in the blood-counting chamber. 

 Adult dogs under morphin and chloroform were used. 



Preliminary determinations, with the animal quiet, showed that 

 for any one individual the number of leukocytes per c.mm. of 

 lymph was practically a constant during the 1-4 hours in which 

 observations were made. Thus certain unavoidable changes in 

 the body state — increased concentration of the blood as the body 

 lymph drained away, variations in the amount of anesthetic — 

 could for later work be ruled out as regards any marked influence 

 in lymph's cell content. 



