Scientific Proceedings. 



— which attack and destroy the integrity of the red corpuscles, and 

 other chemically undefined bodies, — bacterial hemolysins, which 

 act in the same manner, and the more indirect action of certain 

 complexes defined as intermediary body and complement. I have 

 shown previously that certain soaps and fatty acids — of the oleic 

 series, chiefly — can play the part of complements in hemolysis. 

 The experiments based upon this fact led me to the study of the 

 ferment lipase as the direct or indirect cause of hemolysis. I 

 found in the course of this study that lipase is, under some condi- 

 tions, an efficient hemolytic agent which acts, however, not directly 

 upon the red corpuscles, but indirectly through the liberation from 

 available fats of the active fatty acids. Neutral fats, the higher 

 glycerides, are not hemolytic, but they become so under the 

 influence of lipase. 



If one drop of triolein, or a corresponding amount of fat from 

 the dog or guinea-pig, or a small quantity of tripalmitin or crotin, 

 is added to 2 c.c. of a 5 per cent, suspension of washed red cor- 

 puscles and 1 c.c. of the lipase solution be added, hemolysis will 

 occur. Neither the lipase nor the fats alone are lytic. Lecithin 

 cannot replace the fats mentioned. The hemolysis is non-specific. 

 Serum of the dog and the guinea-pig, and, to a less extent, of the 

 ox are rendered non-specifically hemolytic by the action of lipase. 



Potassium cyanide and sodium fluoride in 1 : 10,000 solution 

 inhibit the action of lipase on the fats, and calcium chloride 

 removes the lytic agent from an active mixture. Since the bile 

 salts are known to increase lipolysis, the effects of the sodium salts 

 of cholic, glycocholic and taurocholic acids in 1/500 N solutions 

 were tested on lipolytic hemolysis. The rate of hemolysis was 

 accelerated. 



93 (236) 



On the mechanism by which water is eliminated from the 

 blood capillaries in the active salivary glands. 



By A. J. CARLSON, J. R. GREER and F. C. BECHT. 



[From the Hull Physiological Laboratory of the University of 



Chicago. ] 



I. There is a spontaneous flow of lymph from the quiescent 

 parotid gland of the horse. The quantity is never great but it was 



