132 Society for Experimental Biology and Medicine. 



evident in all of our nine experiments. It is probable that part of 

 the lymph that flows from the neck lymphatics in an anesthetized 

 dog with all the salivary glands at rest comes from the salivary 

 glands. This fact necessitates a limitation of Asher's theory of 

 lymph production. 



2. When the parotid of the horse is thrown into activity by 

 stimulation of the cranial secretory nerves or by injection of pilo- 

 carpi into the blood there is no appreciable increase in the out- 

 put of lymph from the gland as compared with that from the gland 

 at rest. This is true both of the spontaneous flow and of the flow 

 aided by direct massage of the gland. 



3. The activity of the submaxillary does not appreciably 

 influence the flow of lymph from the neck lymphatic in the dog. 

 This conclusion is based on experiments on thirteen dogs. If the 

 activity of the submaxillary gland increases the output of lymph 

 from the neck ducts, the increase is too slight to be detected by our 

 present method, and is not one-tenth of the saliva eliminated by 

 the gland, as Barcroft's observations would seem to demand. Our 

 experiments were made on the spontaneous flow ; on the flow aided 

 by movements of the lower jaw by a mechanical contrivance to 

 secure absolute uniformity in rate and amplitude ; and on the flow 

 aided by direct massage of the head and neck by kneading. More- 

 over a check was introduced by way of recording the lymph flow 

 from both neck ducts, while the submaxillary gland on one side 

 only was thrown into periodic activity by chorda stimulation. As 

 our results are directly contrary to those of Asher and Bainbridge, 

 the question should be reinvestigated by others. 



4. In dogs under light ether anesthesia, perfectly quiescent and 

 with all the salivary glands at rest, there is always a spontaneous 

 flow of lymph from the neck lymphatics. If the anesthesia is 

 pushed till the blood pressure falls considerably, this spontaneous 

 flow ceases. The fact that Asher and Bainbridge worked on dogs 

 under morphin may account for their failure to obtain lymph from 

 the neck ducts in the absence of massage. 



5. The osmotic pressure of the lymph from the active parotid 

 of the horse is not the same in all animals. It may be either the 

 same, higher or lower than that of the serum. In fact in three 

 out of our five experiments it was lower. In four of the experi- 



