Scientific Proceedings. 



157 



emulsion was injected into the peritoneal cavity and the fragments 

 of living tumor are introduced beneath the skin. The promoting 

 effect on the growth of the tumor fragments to be described be- 

 came evident in several sets of experiments in which the same 

 emulsion (unheated), blood serum, bouillon, salt and Ringer 

 solutions were injected in the same manner, with which substances 

 this promoting effect was not obtained. If the inoculation of 

 the fragment of the tumor is made twenty four hours after the in- 

 jection of the unheated emulsion, no difference is noted between 

 the control rats, the rats injected with the other substances, and 

 those injected with heated emulsion. But if the fragments are 

 inoculated ten or more days (up to thirty days) later, then the 

 number of tumors which develop in the rats receiving the heated 

 emulsion tends to exceed the controls and the other series men- 

 tioned ; they grow with greater rapidity so as to reach double the 

 size of the controls or even a still greater size, and show a far 

 smaller percentage of recoveries (retrogressions). This promoting 

 influence is present, as stated, on the tenth day after inoculation, 

 and indications exist tending to show that it is less effective at the 

 expiration of thirty days. On the other hand, indications also 

 exist tending to show that if the injections of heated emulsion are 

 repeated once or twice at ten-day intervals, the conditions of the 

 animal favoring the growth and persistence of the tumors can be 

 maintained and possibly even still further increased. 



no (253) 



On the chemical inactivation and regeneration of complement. 

 By HIDEYO NOGUCHI. 



[From the Rockefeller Institute for Medical Research.^ 

 The complementary substances of an active serum were sup- 

 posed to be extremely labile bodies, but their stability has never 

 been tested chemically. In this study, the action of various acids, 

 alkalies and salts upon complements has been examined. The 

 list of chemicals used is as follows: acids — hydrochloric, nitric, 

 sulphuric, phosphoric, formic, acetic, propionic, lactic, butyric, 

 oxybutyric, oxalic, tartaric, citric, fumaric, maleinic, citraconic, 

 itaconic, glycerophosphoric, uric and nucleic; alkalies — am- 



