38 



Scientific Proceedings (95). 



same individual frog were taken. The Haas measurements were 

 made by putting the tissue to be tested into a small test-tube con- 

 taining 3 c.c. of Ringer's solution tinted with the indicator and 

 previously rendered pH 7.8 with a drop or two of n/10 NaOH to 

 the liter. The tube was closed with a paraffined cork. A bubble 

 of air acted as a stirrer and the preparation was inverted and 

 righted at short intervals until the tint of the solution matched 

 that of a standard tube of solution whose pH = 7.4. Time was 

 taken with a stop watch and the number of seconds required for 

 this amount of change gives an inverse measure of the rate. 



time in seconds ' 



As soon as one reading was taken the tube was emptied of its 

 fluid and refilled from the stock bottle, and another reading taken. 

 As a rule five or six consecutive readings were made. The last 

 two or three were fairly constant; hence their average values are 

 given in the tables and form the basis of the graphs. A set of 

 readings was made each hour and in the interval the tissues re- 

 mained in their respective tubes each containing 3 c.c. of solution 

 (Table I., Fig. 1) or in beakers each containing 100 c.c. of neutral 



Table I. 



Hour. 



Medulla. 



Muscle, 



Sciatic. 



Sciatic 

 Medulla. 



Sciatic 

 Muscle. 



Time. 



Rate. 



Time. 



Rate. 



Time. 



Rate. 



0 



18 



5-5 



20 



5-0 



110 



.91 



.16 



.18 



I 



45 



2.2 



55 



1.8 



180 



•55 



•25 



•30 



2 



80 



1-25 



120 



.83 



360 



.28 



.22 



•34 



3 



85 



1. 17 



150 



•67 



900 



.11 



.09 



.16 



4 



60 



i-7 



40 



2-5 



300 



•33 



Av. - .18 



• 245 



5 



60 



i-7 



60 



1-7 



400 



•25 







6 



90 



1.1 



120 



.83 



600 



• 17 







Table II. 



Hour. 



Medulla. 



Muscle. 



Sciatic. 



Sciatic 

 Medulla. 



Sciatic 

 Muscle. 



Time. 



Rate. 



Time. 



Rate. 



Time. 



Rate. 



0 



30 



3-3 



70 



1-43 



270 



•37 



.11 



.26 



I 



60 



1-7 



90 



I. II 



540 



.185 



.11 



•17 



2 



100 



1.0 



120 



•83 



1000 



.1 



.10 



.12 





120 



•83 



190 



•52 



1200 



.07 



.08 



•13 



4 



145 



.69 



220 



•45 



1200 



.07 



.10 



•15 



18 



300 



•33 



1000 



.10 



1620 



.06 



.18 



(■60) 

















Av. = .11 



•17 



