9 2 



Scientific Proceedings (98). 



plated; the solutions of high concentration, 64.4, 82.8, 100 per 

 cent, were placed in a shaking machine and shaken for 5 minutes. 

 Uniform distribution of the organisms was thus obtained even in 

 the concentrated solutions. 



The average results of 23 different series of tests are shown for 

 typical time intervals in the table above. They suggest the 

 following conclusions. 



1. Glycerol in 9 per cent, solution exerts no appreciable effect 

 upon the viability of B. coli. 



2. Glycerol in solutions of strengths between 28 per cent, and 

 100 per cent, exerts a distinct disinfectant action, the effect 

 increasing progressively with increase in the concentration, a 100 

 per cent, solution of glycerol causing the destruction of nine 

 tenths of the bacteria present in three hours. 



53 (1428) 



The effects of intravenous injections of dichlorethylsulphide 



in rabbits. 



By Alwin M. Pappenheimer. 



[From Columbia University, New York City.] 



The effect of intravenous injections of dichlorethylsulphide 

 (mustard gas) was studied in a small series of rabbits. The mini- 

 mum lethal dose was found to be from 0.005 g m - to 0.01 gm. per 

 kilo. The injection was followed by emaciation, diarrhea, and, 

 in animals dying within a few hours following the injection, ex- 

 treme restlessness, incoordinate movements, retraction of the 

 head, and transient spasticity, but no definite paralyses or con- 

 vulsions. Animals dying within twenty-four hours or so showed 

 irregular pulmonary edema. The most interesting effects were 

 found in the hemato-poietic system. Usually on the second 

 day after the injection, the circulating blood showed a marked 

 leukopenia, which in the terminal stages became extreme, leuko- 

 cytes falling to 1,000 per cubic mm. or less. In animals which 

 recovered there followed a gradual restoration to the original 

 level. The leukopenia was accompanied by a relative but not 

 absolute, mononucleosis. The erythrocytes appeared to be less 

 severely injured. 



