Non-protein Sulphur of the Blood. 139 



Unlike the effects of thyroid feeding, the feeding of prostatic 

 substance while hastening metamorphosis did not produce much 

 shrinkage in the size of the tadpoles. Indeed, it very often seemed 

 to promote the growth of the tadpoles to a greater degree than 

 was noted in the control animals. It was further noted, that 

 prostatic substance was very much less toxic to the larvse than 

 was thyroid substance, so that the tadpoles could be fed on the 

 prostate continuously without being killed. All kinds of control 

 experiments with various glands and other tissues were made and, 

 as a result of these, it was definitely established that the inter- 

 esting effect on the metamorphosis of tadpoles was not produced 

 by any other tissue except the thyroid and the prostate. A more 

 extensive study on the feeding of prostatic substances of various 

 animals to tadpoles, rats, and other animals is in progress and will 

 be reported in due time in the Journal of Urology. 



78 (1453) 



Non-protein sulphur of the blood: Its determination, its frac- 

 tionation, and its clinical significance. 



By Max Kahn. 



[From the Department of Laboratories, Beth Israel Hospital.] 



Sodium citrate was used as anticoagulant. The protein of 

 the plasma was precipitated by acetone — free methyl alcohol and 

 zinc chloride (c.p.). The total sulphur was determined by oxida- 

 tion with potassium chlorate and precipitation with barium 

 chloride. The total sulphate was estimated by a method similar 

 to the one of Vansteenberghe and Bauzil. The neutral sulphur 

 was computed by subtracting the total sulphate from the total 

 sulphur. 



Studies were made on the blood of patients suffering with 

 kidney, liver and malignant disease as well as those suffering with 

 chronic infections. 



