i8 



Scientific Proceedings (ioi). 



at the stage of the first maturation spindles; this gave rise to the 

 X6 series with evidence of partial rejuvenescence. The third 

 pair was cut at the stage of the second maturation division and 

 this gave rise to the X7 series which showed the same rejuvenes- 

 cence as the normal ex-conjugant from the same source. 



Many more experiments of the same nature are now under 

 way and must be carried out before conclusions can be drawn. 

 These three cases indicate, however, that the absorption of 

 nucleo-proteins in the cytoplasm and which occurred in all cases, 

 is not, by itself at least, the secret of renewed vitality. 



8 (1468) 



The total carbonate content of the arterial and venous plasma in 

 normal individuals. 



By R. W. SCOTT (by invitation). 



[From the Department of Medicine, School of Medicine, Western 

 Reserve University, Cleveland.] 



In the course of some observations on the respiratory dis- 

 turbances seen in certain diseased conditions it became necessary 

 for the sake of comparison to determine the total carbon dioxide 

 of the arterial and venous plasma in individuals with normal 

 cardio-respiratory mechanisms. In all the bloods of nineteen 

 normal individuals at rest have been examined. In each case 

 samples of arterial and venous blood were obtained within a few 

 minutes of each other. 



Method. — The arterial blood was obtained by direct puncture 

 of the radial artery using a technique similar to that employed 

 by Stadie. 1 The venous blood was collected without stasis from 

 one of the large veins at the bend of the elbow. To avoid con- 

 tact with air both samples were carefully delivered under albolene 

 into paraffin coated centrifuge tubes and immediately centrifuged 

 at high speed. One c.c. of the separated plasma was delivered 

 under carbonate free ammonia water contained in a receiving cup 



1 Stadie, W. C, J. Exp. Med., 1919, XXX, 215. 



