54 



Scientific Proceedings (103). 



Experiment 2. To determine the effect of variation in the concentration of the 

 dextrine vitamine solution. 





0.1/Cc. Dextrine V. 



0.2/Cc. Dextrine V. 



Sterilization Method 



Arnold. 



Arnold Plus 

 Autoclave. 



Arnold. 



Arnold Plus 

 Autoclave. 



Series 



E, 



2. 



3. 





2. 



3- 





2. 



3- 



1. 



- 



3- 



Days: 



























1 



0 



0 



0 



0 



0 



0 



0 



0 



0 



0 



0 



0 



2 



0 



0 



0 



0 



0 



0 



16 



2 



I 



50 



0 



0 



1 . . 



0 



0 



0 



0 



0 



0 



63 



35 



78 



70 



18 



75 



4 



0 



0 



0 



0 



0 



0 



29 



25 



99 



35 



30 



67 





0 







0 







II 







72 







7 



0 



1 



3 



0 



0 



0 



10 



8 



79 



62 



7 



22 



8 



0 







0 









5 





52 







9 



0 







0 







4 







48 







14 



0 







0 







3 







45 







Tube Contents 



0.5/Cc. Dextrine V. 



i.o/Cc. Dextrine V. 





Arnold 





Arnold Plus 

 Autoclave. 



Arnolc 





Arnold Plus 

 Autoclave. 



Series 



1. 



2. 



3- 



1. 



2. 



3- 



1. 



2. 



3- 



1. 



2. 



3- 



Days: 





























0 



0 



0 



0 



0 



0 



0 



0 



0 



0 



0 



0 



2 



95 



95 



53 



26 



60 



23 



90 



99 



68 



93 



99 



68 



3 



9i 



100 



95 



51 



100 



100 



87 



100 



100 



85 



100 



100 



4 



85 



99 



95 



58 



84 



96 



63 



75 



82 



58 



65 



80 



5 



68 







25 







18 







56 







7 



10 



10 



10 



8 



12 



8 



40 



20 



8 



u 



30 



28 



8 



8 







7 







38 







u 







9 



8 







7 







37 







u 







14 



7 







6 







33 







u 







Explanation: The only variants were the amounts of vitamine solution used and 

 the yeast suspensions. 



All the Vitamine solutions were sterilized as in experiment 1 and the second set in 

 the Autoclave for additional 20 minutes. In series 2 and 3 a new yeast suspension 

 was made. The Vitamine solution was the same as used in Experiment 1. 



