The "Bachman Test." 



55 



Experiment 3. To determine the suitability of the test as a qualitative agent in 

 determining the presence and relative amount of "B" Vitamine. 



N.B. All tubes contained the Nageli solution and a loopful of yeast suspension 

 as in Experiments 1 and 2. In this experiment only Arnold sterilization was used 

 (two 30-minute periods with 24 hours incubation period intervening). 



Tube Contents. 



Per Cent, of Gas Formed by 

 Days. 



1. i c.c. of a vitamine extract of a commerical farina. 

 110 gms. farina was used and the extract made up to 

 150 c.c 



2. Vitamine extract from 25 gms. alfalfa. Extract made 

 up to 150 c.c. Using 0.5 c.c 



Using 1 c.c 



4. Protozoa food mixture after Calkins made by boiling 

 130 gms. flour with 100 gms. timothy hay and 100 

 c.c. distilled water, allowing to cool and decanting. 



Used 0.5 c.c 



Using 1.0 c.c 



5. 35 c.c. of the Protein free milk obtained from 1 qt. 

 milk 



6. 1 c.c. Walker Gordon whole milk 



7. Duplicate of (6) with varying concentrations: 



Using 1 c.c 



Using 0.5 c.c 



Using 0.3 c.c 



8. Cow's milk to which was added varying amounts of 

 N(NaOH) solution before sterilizing. 



Using 1 c.c. of solution (25 c.c. milk cont. 1 c.c. 



N(NaOH) ) 



Using 1 c.c. of solution (25 c.c. milk cont. 0.5 c.c. 



N(NaOH) ) 



Using 1 c.c. of solution (25 c.c. milk cont. 0.4 c.c. 



N(NaOH) ) 



Using 1 c.c. of solution (25 c.c. milk cont. 0.3 c.c. 



N(NaOH) ) 



Using 1 c.c. of solution (25 c.c. milk cont. 0.2 c.c. 



N(NaOH) ) 



Using 1 c.c. of solution (25 c.c. milk cont. 0.1 c.c. 



N(NaOH) ) 



o 

 o 



20 

 100 



68 

 52 

 25 



83 



o 



15 



40 



100 



100 

 100 



60 



70 



58 



100 

 100 

 100 

 100 



o 



32 



53 

 100 



100 



88 

 50 



100 

 95 

 100 

 100 

 100 

 100 



33 



48 



28 



60 



9. Breast milk from six different sources. Each specimen used sterilized two 30- 

 minute periods in the Arnold Sterilizer before using. 1 c.c. used in each inocula- 

 tion. 3 series of experiments. Series 1 used a different yeast suspension from 

 that used for Series 2 and 3. 



Sources. 



Series i. 



Sources. 



Series 2. 



Sources. 





Series 



3« 







2. 



3« 



4- 



5. 



6. 



7. 



X. 



2 . 



3- 



4' 



5- 



z. 



2. 



3- 



4- 



5. 



A. 1 c.c. . . 



0 



12 



63 



80 



100 



100 



100 



I C.C. 



0 



0 



60 



100 68 



0.5 C.C. 



0 



0 



0 



10 



30 



B. 1 c.c. . . 



O 



21 



59 



70 



70 



65 



65 



I C.C. 



O 



31 



70 



72 62 



0.5 C.C. 



0 



0 



24 



65 



90 



C. 1 c.c. . . 



0 



17 



47 



68 



82 



68 



65 



I C.C. 



0 





82 



60 65 



0.5 c.c. 



0 



3 



33 



50 



48 



D. 1 c.c. . . 



0 



21 



72 



72 



72 



68 



68 



I c.c. 



0 



65 



90 



80 70 



0.5 c.c. 



0 



0 



28 



78 



90 



E. 1 c.c. . . 



0 



I 



23 



56 



65 



45 



44 



I c.c. 



0 



5 



70 



9065 



0.5 c.c. 



0 



0 



20 



68 



64 



F. 1 c.c. . . 



0 



0 



20 



35 



59 



55 



54 



I c.c. 



0 



3 



78 



72l45 



0.5 c.c. 



0 



0 



48 



68 



50 



