Phenol Elimination in the Dog. 



105 



detoxication is subnormal, owing to injury sustained during the 

 course of the infection by the hepatic, renal and other cells con- 

 cerned in this mechanism. Of course, normal variations in this 

 process cannot be overlooked as a possible factor. 



We have, therefore, studied, in a preliminary way, the elimi- 

 nation of free and conjugated urinary phenols after the intravenous 

 injection of a single large tolerated dose (50 mg. per kilo) of 

 neo-arsphenamine into the dog. 



Method. — A normal healthy female dog (16.82 kilo) was 

 employed and was kept on a constant diet. The technique 

 followed was that of Folin and Denis 1 with the modifications 

 described by Dubin. 2 



Results. — The results are summarized in Table 1 and indicate 

 the following: 



TABLE I. 



Phenol Elimination after a Single Intravenous Injection of Neo- 

 arsphenamine (0.841 Gr.). 1 





Control 



1st Neo 



2d Neo 



3d Neo 





Summary. 











Period. 



Period. 



Period. 



Period. 













Daily Elimi- 































nation 



u 









V 



3 



V 













V 



? 





V 



C4 



O 



1 



rt 



O 



V 



0 



V 



rt 

 O 



Periods of Exp. 



*> 



S 

 u 



0 



'c? 

 0 



fa 









H 





H 



& 



H 





H 



fa 



H 



U 



68 







106 



156 



117 



158 



95 



147 



90 



127 



Normal 



528 



734 



206 



72 



28 



2d M 



117 



187 



96 



125 



US 



173 



108 



143 



1st neo 



506 



673 



167 



75 



25 



3d 11 



78 



no 



82 



109 



105 



140 



119 



173 



2d neo 



581 



875 



294 



66 



34 





















3d neo 



5" 



728 



217 



70 



30 



4th " 



106 



149 



106 



143 



152 



238 



88 



138 



Total 3 neo 













5th " 



















periods 



1.598 



2,276 



678 



70 



30 



121 



132 



105 



138 



114 



177 



106 



147 



15 days 































"Extra" 













Total 5 days 



528 



734 



506 



673 



58i 



875 



5ii 



728 



phenols 



14 



74 



60 



19 



81 





















15 days 













All figures refer to miligrams unless otherwise indicated. 



1. The elimination of "normal" free and conjugated phenols 

 is quite uniform from day to day, if the animal is kept on a con- 

 stant diet. The work of Folin and Denis is thus confirmed. 



2. After a single intravenous injection of neo-arsphenamine, no 

 appreciable variation in phenol elimination is observed during 

 the first period of five days. During the next period there is an 

 increase in both the free and conjugated phenols. During the 



1 Folin, O., and Denis, W., Jour. Biol. Chem., 1915, 22, 305-308. 



2 Dubin, Harry, Jour. Biol. Chem., 1916, 26, 69-91. 



