Phenol Elimination of the Dog. 107 



Since the administration of sulphates is known to counteract 

 the effects of phenol poisoning, and particularly in view of the 

 work of Bufalini, 1 we studied the phenol elimination in the 

 same dog after a single intravenous dose of neo-arsphenamine 

 during the time that she was receiving daily subcutaneous injec- 

 tions of ammonium persulphate. 



Method. The animal was kept on the same constant diet. 

 Phenol elimination was studied for five days (control period) ; 

 ammonium persulphate was now given subcutaneously twice 

 daily in 2.5 per cent, solution in physiological saline in doses of 

 10 c.c. and phenol excretion was measured during this period. 

 A single intravenous dose of 0.841 g. of neo-arsphenamine was 

 now given and persulphate injections and estimation of urinary 

 phenols was continued for ten more days. 



Results are summarized in Table 2, and indicate the following: 



1. The subcutaneous injection of ammonium persulphate 

 which theoretically would furnish the organism with additional 

 sulphuric acid for phenol conjugation, does not result in the elimi- 

 nation of any "extra" conjugated "normal" phenols. During a 

 period of five days, only a slight increase in the free "normal" 

 phenols was observed. 



2. After the intravenous injection of a single dose of 0.841 

 g. of neo-arsphenamine and the daily subcutaneous administration 

 of the persulphate, an enormous increase in the output of "extra" 

 free phenols was observed. Simultaneously, a marked diminution 

 in the excretion of "extra" conjugated phenols was noted. The 

 animal also showed definite symptoms of intoxication. We 

 must, therefore, conclude that the "extra" free phenols were due 

 entirely to tissue destruction resulting from the toxic effects of 

 the persulphate. 



The writer is indebted to Dr. John A. Kolmer, Head of the 

 Department of Pathology, for his encouragement and unfailing 

 kindness throughout the course of this work. 



1 Bufalini, G., Archives ital. de Biol,, 1903, 40, 131-140. 



