n8 



Scientific Proceedings (106). 



The pathologic lesion of acne is in the sebaceous glands of 

 the hair follicles, while in furunculosis the sweat glands become 

 infected. It is of interest, then, to note that in one patient with 

 folliculitis barbae, in which the hair follicle is involved, the amount 

 of calcium in the blood was increased. 



Gravimetric Method Used. 

 The blood was collected in a flask containing 90 mg. of sodium 

 citrate. After centrif ugalization the plasma was drawn off and 

 evaporated in a platinum dish; ashed with addition of nitric acid. 

 The ash was taken up with dilute hydrochloric acid. The solution 

 was made alkaline with ammonia and then faintly acidulated 

 with acetic acid. The iron was precipitated with 20 per cent, 

 sodium acetate. The calcium was precipitated from the filtrate 

 with 4 per cent, ammonium oxalate. The solution was allowed to 

 stand over night. Gooch crucibles packed with asbestos were 

 used. The calcium was weighed as calcium oxide. 



66 (1526) 



The preparation of animal nucleic acid. 



By Emil J. Baumann. 



[From the Chemical Laboratory of the Montefiore Home and Hospital, 



New York City.] 



The proposed method depends on the following properties of 

 nucleic acid: (1) that it is separated from its protein combination 

 by treatment with sodium hydroxide, (2) that it is soluble in 

 dilute acetic acid and, (3) that it is precipitated by hydrochloric 

 acid, or in the case of dilute solutions, by hydrochloric acid in the 

 presence of magnesium sulphate. 



Fresh, trimmed glandular tissue, finely hashed, is mixed well 

 with twice its weight of tap water and 100 c.c. of 50 per cent, 

 sodium hydroxide is then added for each kilogram of tissue. 

 The material is then heated until all except the connective tissue 

 is dissolved (40 0 C.-70 0 C.). Neutralize hot and at once with 

 strong acetic acid; the reaction should finally be distinctly acid to 

 litmus. Bring to a boil and filter hot on large folder filters. 



When the filtrate has cooled an aliquot of 200 c.c. is taken and 



