122 Scientific Proceedings (106). 



is correlated with a parallel rise in corpuscular volume as indicated 

 in the table. 



The concentration of sugar falls steadily throughout the work 

 period. It should be noted, however, that the variations during 

 the first period in work and those during the same period in rest 

 are almost identical, but from this point on there is a divergence 

 which becomes quite pronounced in the later stages of the expe- 

 riment. 



It is perhaps worthy of notice that there is a rather distinct 

 change in the magnitude of the effects of work after the first 

 period. This we have interpreted as indicating that the day's 

 work falls into at least two phases; first, one in which certain 

 augmenting effects of exercise apparently predominate, second, 

 one in which fatigue phenomena are relatively more prominent. 



69 (1529) 



Further studies in the measurement of vitamine content. 

 By Walter H. Eddy and Helen C. Stevenson. 



[From the Department of Physiology Chemistry, Teachers College, 

 Columbia University and the Department of Pathology, New 

 York Hospital.] 



During the past year two papers have appeared in the Journal 

 of Biological Chemistry, one by Dr. Bachman 1 and the other by 

 Dr. Williams 2 , which postulate the requirement of the water- 

 soluble B vitamine in yeast growth. Both these papers suggest 

 the use of yeast cells as a means for the measurement of vitamine 

 content and present a technique that may be used to that end. 



In an earlier number of the Proceedings 3 we reported on a 

 study of the suitability of the Bachman test for vitamine measure- 

 ment and some of the difficulties encountered. Since that time 

 we have carried out similar studies of the Williams technique and as 

 a result have devised a method which employs features of both 



1 Bachman F., Vitamine requirements of certain yeasts. Journ. Biol. Chemistry 

 1919, xxxix, 235. 



2 Williams, Roger J., The vitamine requirement for yeast. A simple biological 

 test for vitamine. Journ. Biol. Chem., 1919, xxxviii, 465. 



3 Proceedings Soc. Exper. Biol, and Med., 1919, xvii, 52. 



