Formation of Antibodies. 



133 



nitrogen analyzed immediately after removal of the tubes from 

 the press and "48 hrs.," that nitrogen found in aliquots after 

 remaining in a thermostat at 37 0 for that period. 



Sample Number. 



Pressure. 



Time. 



Initial. 



48 Hours. 





XX 



XX 



0.50 c.c. 0.1 N. 



0.80 C.C. O.I N. 





75(io) 8 



5 mins. 





.90 





16(10)4 



2 hrs. 





•50 





do) 6 



16 hrs. 





1.40 





do) 5 



1 min. 





•50 



The explanation of number III being at variance with number 

 IV, where both are at the same degree of compression, doubtless 

 lies in the fact that during the longer period of exposure (16 hrs.), 

 some enzyme action may proceed before inhibition sets in, espe- 

 cially as the temperature rises somewhat 4 during this prolonged 

 time of exposure. 



It is evident that the method can be used only with the greatest 

 care to adjust the pressure so that complete inhibition does not 

 occur, while at the same time, all bacteria 5 are killed, which is of 

 doubtful practicability. 



At these pressures, egg albumen is coagulated (B.H.H.), but 

 in the case of the tissue from the rabbit's liver, there is little 

 evidence of change in the colloidal dispersion, although the 

 Ringer's Solution used to make up the liver mass to 20 per cent, 

 suspension separates in the tubes during compression from the 

 semi-solid mass. 



73 (1533) 



The relation between the disappearance of foreign proteins from 

 the circulation and the formation of antibodies. 



By Warfield T. Longcope and George M. Mackenzie. 



[From the Presbyterian Hospital, New York City.] 



Some observations which Dr. Rackemann and one of us made 

 a few years ago indicated that when serum disease followed the 

 injection of horse serum in human beings anti-bodies, such as 



4 The cylinder is cooled during the period of compression. 

 6 This probably lies near (10). 3 



