Coli Fever and Blood Volume in Dogs. 



149 



0.6° C. A third strength of vaccine used contained one million 

 bacilli per c.c; doses of y£ and 1 c.c. per kilo, gave maximum 

 increases of i.o° and 1.2 0 C. respectively. In all of the experi- 

 ments with the last two vaccines the maximum temperature was 

 attained by either the second or the third hour after the sub- 

 cutaneous injection. At the end of the day (6-8 hours after in- 

 jection) the temperature elevation was usually reduced by about 

 one half. 



The last two strengths of vaccine mentioned were therefore 

 considered suitable for further studies. On the second day the 

 temperature was usually found somewhat elevated, but thereafter 

 not at all. The dogs could not as a rule be used for more than one 

 injection each as repetition of the same or larger doses after four 

 days gave a less pronounced effect, indicating that some degree of 

 immunity had been produced. 



In all experiments the injections gave sterile abscesses de- 

 veloping to a considerable size and breaking down several days 

 after the injection. About half of our dogs were fasted for two 

 days before being injected, the others being kept on a constant 

 adequate diet of meat, lard, and bread; water ad libitum was 

 always allowed. No essential differences were noted between 

 fed and fasted animals. 



t 



Coli (#5) 1 c.c. per kilo 

 38 180, , , , | | I 



Hours 10 12 2 4 



Fig. A. Dog 47. Jan. 3. (Fasted 2 days.) 



blood solids. 



rect. temp. 



