154 



Scientific Proceedings (107). 



As a control to the above experiments, intravenous injections 

 were made of 0.9 per cent, sodium chloride, of which was given 

 8 c.c. per kilo to each of two dogs exhibiting a typical coli fever, 

 in the first fifteen minutes there was observed no change whatever 

 in the temperature which subsequently ascended to points 0.7 0 C. 

 and 0.4 0 C. respectively higher than the former febrile level. 

 The blood solids on the other hand showed a slight diminution 

 after half an hour, and the hemoglobin fell within the first quarter 

 of an hour. (See fig. 3). The control experiments therefore 

 showed that isotonic NaCI in amounts comparable to the dex- 

 trose injections is not able to reduce the temperature of coli fever 

 dogs, nor is the increase in blood volume as marked as when 

 the dextrose was given either to the normal or the fever dogs. 



It is therefore concluded that the antipyretic effect (not 

 noted in health) of intravenous dextrose injections is due to 

 osmotic action by which in fever dogs an unusually profound 

 increase in the fluids of the blood results for a short time. This 

 increase in fluid is of value to the animal in promoting heat elimi- 

 nation. The tissues in coli fever appear to contain a higher 

 percentage of "available water" than is normally present. Sen- 

 sitivity to antipyretic drugs can thus be accounted for. 



81 (1541) 



Preparation and refining of diphtheria toxin-antitoxin. 

 By Edwin J. Banzhaf. 



[From the Bureau of Laboratories, Department of Health.} 



The mixture has been readjusted so that after standing eight 

 months and longer, late paralysis will occur when the mixture 

 is injected into guinea pigs. This is done by adding to each 

 L-f- dose one unit of a properly aged antitoxin. When five mils 

 of this mixture is injected into guinea pigs acute death occurs 

 within four or five days. The mixture is then stored in a refriger- 

 ator for a month to six weeks for stabilizing. 



On reinjecting five mils, after storage, the guinea pigs die of 

 late paralysis after twenty to twenty-five days. It is then properly 



