Scientific Proceedings (107). 



and 13 were held as controls. Generalized lesions developed in 

 8 of the 13 controls and 13 of the 14 castrated animals. 



Of 20 rabbits inoculated in both testicles, 6 were held as 

 controls and 14 were castrated. Generalized lesions occurred in I 

 of the 6 controls and in 13 of the 14 castrated animals. 



Several other experiments of a similar character gave essen- 

 tially the same results. In one of these, 46 rabbits were given a 

 heavy inoculation with a testicular emulsion — half of them uni- 

 laterally and the other half bilaterally. With these animals, the 

 influence of castration at different periods of the infection was 

 studied and the effects of suppression of the local infection by the 

 use of a therapeutic agent. Only the results of the therapeutic 

 experiments can be given here. 



Effects of Suppression of Primary Lesions by Therapeutic 

 Agents. — In carrying out these experiments, a drug was chosen 

 from among those studied by us in collaboration with Dr. W. A. 

 Jacobs and Dr. Michael Heidelberger whose effect in inducing 

 resolution of lesions was much greater than its spirocheticidal 

 action. This substance was arsenophenylglycyl dichloro-ra- 

 aminophenol. 



Twelve rabbits, 6 of them inoculated unilaterally and 6 bilater- 

 ally, were given a single intravenous injection of this drug 14 days 

 after inoculation and the results were controlled by 6 untreated 

 rabbits from each of the respective groups. 



In the unilateral series, the lesions present were almost com- 

 pletely resolved and the local reaction suppressed for between 

 2 and 3 weeks. At the end of 3 months, all of these animals had 

 developed generalized lesions as contrasted with 3 of the 6 con- 

 trols. The effect of the drug upon the animals inoculated in 

 both testicles was less marked and lasted for only 7 to 10 days. 

 At the end of 3 months, generalized lesions had developed in 4 of 

 the 6 treated animals and in I of the 5 surviving controls. 



Effects of Complete Prevention of a Primary Reaction and 

 Early Removal of the Medium of Inoculation. — An experiment 

 originally carried out for the purpose of determining the time at 

 which a true generalized infection became established in the 

 rabbit proved to be a remarkable demonstration of the effects 

 which might be obtained from complete prevention of the develop- 



