Intraventricular Heart Block. 



177 



from the vitamine fraction from yeast in 191 2 and 191 3 were 

 tested. Those of 1913 were analyzed at that time and their 

 formulas established. These substances showed an attenuated 

 but definite activity. Polished rice was entirely negative as 

 opposed to the statement of H. H. Green. Saliva was found 

 inactive and urine as already reported by Muckenfuss was active. 

 The latter test may prove of clinical value later on, but possibly 

 blood could be used to greater advantage for diagnostic purposes. 

 A few discrepancies were found however; for instance, yeast 

 treated with Lloyd's reagent still retained a large portion of its 

 activity and also it was found that corn and wheat, separated 

 from the germ by the method of Voegtlin and Myers, still exhibited 

 a large activity, although several times less than the germ con- 

 taining portion. Further experimentation is under way to clear 

 up these matters. 



By comparing the method of extraction of Osborne and Wake- 

 man from yeast with an extract prepared from autolyzed yeast 

 and then heated over 50 0 to coagulate proteins (we found some 

 heat coagulable protein in autolyzed yeast) we found that Osborne's 

 preparation contains only one-fifth of the nitrogenous substances 

 but exhibits only one-fifth of the activity of the autolyzed yeast 

 extract. It remains to be seen therefore whether it is advisable to 

 use very purified extracts for vitamine fractionations, since it 

 appears that the concentration of vitamines in such crude extracts 

 as above varies with the amount of impurities present. 



94 (1554) 



The site of the cardiac lesion in two instances of intraventricular 



heart block. 



By B. S. Oppenheimer and H. E. B. Pardee. 



[From the Physiological and the Pathological Laboratories, Columbia 



University.] 



The hearts of two cases were examined in order to determine 

 the site of the lesion associated with electrocardiograms suggesting 

 partial block of either the right or the left branch of the auriculo- 



