Purification and Concentration of Antigens. 189 



derivative show quite different ability to lower the blood uric 

 acid in different individuals, the action being very pronounced 

 in some cases and comparatively slight in others. 



In their experiments Folin and Lyman noted the interesting 

 fact (in two cases) that cinchophen not only brings about a 

 dimunition of the uric acid in the blood but also seems to lead 

 to a dimunition of the nonprotein nitrogen and urea whenever 

 these are present in the blood in unusual amounts. Unfortunately 

 these two experiments were not followed by a control after period. 



Data are given in the table above on two cases in which we 

 believe there is unmistakable evidence of a drop in the blood 

 urea as well as in the uric acid. It will be noted that the action 

 on the uric acid was very pronounced in both cases, the first case 

 having a high and the second a normal initial uric acid. 



100 (1560) 



The purification and concentration of antigens by new methods of 



adsorption. 



By Augustus B. Wadsworth and Frank Maltaner. 



[From the Division of Laboratories and Research, New York State 

 Department of Health, Albany.] 



The results of recent studies of complement fixation in tuber- 

 culosis suggest that the test might be of considerable diagnostic 

 value if satisfactory antigens could be prepared. 



Three strains of tubercle bacilli were selected: one a virulent, 

 another a non-virulent, human strain and a third bovine strain. 

 Immune serum sufficient for titration with all the antigens was 

 obtained from inoculated horses. 



The antigens were prepared from cultures by various methods 

 of fractioning and extraction similar to those used by other ob- 

 servers. The culture filtrates were so anticomplementary that 

 they could rarely be used. The glycerine and distilled water 

 extracts gave the most active antigens. These active antigens 

 and also the culture filtrates for comparison were selected for 

 further study by methods of adsorption. 



