Cow's Milk in Infants 



7 



Tests for antigen were made by adding 0.1 c.c of anti-milk 

 rabbit serum too .1 c.c. of the serum to be tested diluted to 1 c.c. 

 with normal saline solution. 



The following control tests were used : 



(1) One-tenth c.c. of the serum tested was diluted to 

 1 c.c. with normal saline. 



(2) One-tenth c.c. of the anti-serum was diluted to 1 c.c. 

 with saline. 



(3) One-tenth c.c. of normal rabbit serum was added to 

 0.1 c.c. of the serum tested diluted to 1 c.c. with saline. 



(4) One-tenth c.c. of normal human serum was diluted 

 to 1 c.c. with normal saline solution and 0.1 c.c. of the anti- 

 milk serum added. 



The controls were uniformly negative and all were not used 

 in each series of tests. In the later tests only 1 and 2 were used. 



The anaphylactic tests for antigen were made by injecting 

 3 c.c. of the citrated blood into the peritoneal cavity of a 200 

 to 300 gram guinea pig. Three weeks later an intoxicating 

 dose of 0.5 to 0.75 c.c. of milk was injected intravenously. 



Observations were conducted on 37 patients with athrepsia 

 and in 24 the precipitine tests were positive on at least one 

 occasion. In many cases the test was repeated from one to 

 eight times. 



In 13 of the cases with positive precipitine tests the attempt 

 was made passively to sensitize guinea pigs by injections of the 

 patients' blood. The results were positive in 8 cases. The re- 

 sults were considered positive only when the animals developed 

 very severe symptoms. When the symptoms were slight or 

 open to any question the experiment was considered negative 

 or was excluded. In the tests considered positive the animals 

 developed marked dyspnea, convulsive movements or paralysis 

 and in most instances died within 15 minutes after the injec- 

 tion. The necropsies disclosed marked distension of the lungs. 



Throughout the investigation many control tests were made. 

 Intravenous injections of milk in the quantities used caused no 

 symptoms in normal guinea pigs. All of the positive experiments 

 on animals were in cases in which the blood showed precipitines 

 for cow's milk. In no instance did the blood of infants not 

 showing precipitines for cow's milk passively sensitize guinea 



