24 



Scientific Proceedings (125) 



tions. As a rule, the animals died three or four days after inocu- 

 lation, about 50 per cent, of them showing a septicaemia of 

 B. influenzae. In this series, all the animals, eight in number, 

 so inoculated, died, but one. At autopsy the usual picture was 

 marked fibrinous pleuresy with a pleural exudate of cloudy fluid; 

 the lungs in two animals showed pneumonia, in the remainder 

 they were apparently negative ; in five cases there was a fibrinous 

 exudate covering the trachea and extending down into the sup. 

 mediastinum. One animal showed a serofibrinous peritonitis. 

 All other organs were apparently negative. Microscopically 

 the lungs from five animal? were examined ; two with pneumonia 

 in gross, and three apparently negative. However, of these 

 three, two showed small focal areas of pneumonia microscop- 

 ically. One case also showed necrosis of the heart muscle fibres. 

 Smears of the pleural exudates showed very numerous organ- 

 isms and but a few leucocytes. The organisms were extremely 

 small and coccoid in shape. Cultures were positive from the 

 pleural exudate and neck exudate, and in about 70 per cent, from 

 the heart's blood. One animal was injected by mistake in the 

 neck tissues instead of into the trachea and died overnight with 

 a septicaemia. This would tend to settle the question as to 

 whether we were not causing the above lesions by missing the 

 trachea and injecting into the neck, for death in these animals 

 where we felt sure we were in the trachea did not occur for 

 three or four days, never overnight as in this animal. In all 

 instances the animal was anesthesized and the trachea exposed 

 by operation. 



2. Intracerebral. Three animals were inoculted with the fol- 

 lowing doses : 0.005 ex. of the pleural exudate of a rabbit killed 

 by intracheal inoculation, 0.001 c.c. of the peritoneal exudate 

 of a rabbit inoculated intraperitoneally, and 0.1 c.c. of a 20 hour 

 chocolate broth culture. Blood cultures on two of the rabbits 

 taken 3*^ hours and five hours, respectively, after inoculation 

 were positive; the third animal was not cultured before death. 

 At autopsy cultures of the heart's blood in all three were posi- 

 tive. Pathologically, nothing abnormal was found with the 

 exception of haemorrhage into the thymus in one animal. 



3. By stomach tube. Five animals were inoculated in the 

 stomach by means of a stomach tube. Of these, two died, one 

 receiving 15 c.c. of 20 per cent, chocolate broth culture, and the 



