26 



Scientific Proceedings (125) 



III. The three strains worked with in this group were "R," 

 a meningeal strain from the spinal fluid of a case of influenzal 

 meningitis; "S," isolated from the sputum of an acute case of 

 influenza; "J," isolated from the pus of a case of chronic infec- 

 tion of the antrum, and "Z," from the sputum of a case of acute 

 coryza. 



A. The following experiments were done with "R" : 



1. Intracerebral. One rabbit was injected with 0.1 c.c. of 

 spinal fluid from a case of influenzal meningitis shortly after 

 removal from the patient. Blood culture on the animal next day 

 was negative. He never was sick, and at the end of three days 

 was used in another experiment. 



Another rabbit was injected with 0.1 c.c. of the peritoneal 

 exudate of rabbit killed by intraperitoneal injection with a 

 chocolate broth culture of this strain. Blood cultures 24 hours 

 later showed approximately 500-1000 colonies in a drop of 

 blood. Blood 48 hours after injection showed 10 colonies to a 

 drop of blood. Blood culture 72 hours and 96 hours after injec- 

 tion were negative. The animal died 10 days after inoculation; 

 at autopsy, the brain showed a yellowish caseous area at the 

 inoculation site, 0.2 x 0.2 x 0.1 cm.; cultures from this lesion 

 and the heart's blood were negative. 



B. The following experiments were done with strain "S" : 



L Intratracheal. One rabbit received 2.5 c.c. of the patient's 

 sputum; it never was sick and blood cultures were uniformly 

 negative. The animal was killed with ether 9 days after; 

 autopsy findings and cultures from the lungs and heart's blood 

 were negative. 



2. Intracerebral. Four rabbits were injected. 



(a) Received first 0.75 c.c. of filtrate of "S" on chocolate 

 broth intravenously with symptoms typical of poisoning with 

 filtrates of cultures of B. influenza ( 1 ) ; while still sick, one and 

 a half hours later he received intracerebrally 0.1 c.c. of the 

 centrifuged sediment of a 20 hour chocolate broth culture. Blood 

 culture 6 hours later and 24 hours later was negative. It died 

 the night of the second day. At autopsy, the left lateral ven- 

 tricle showed on its inferior surface a few yellowish spots and 

 injection of its upper surface; the rest of the organs were nega- 

 tive; smears and cultures of the fluid in the ventricles were 

 positive while the heart's blood were negative. 



