66 



Scientific Proceedings (125) 



29 (1989) 



Influence of pancreatic perfusates upon the carbohydrate meta- 

 bolism of depancreatized animals. 



By HARRY D. CLOUGH, ARTHUR M. STOKES, C. B. F. GIBBS, NEIL C. 



STONE and JOHN R. MURLIN. 



[From the Physiological Laboratory of the University of Roches- 

 ter, Rochester, N. Y.] 



Following the method of perfusion introduced by Clark 1 , the 

 effect of pancreatic perfusates upon the carbohydrate metabolism 

 of entire animals was studied. The respiratory metabolism, the 

 blood sugar, and the D :N ratio were studied in cats and dogs 

 after depancreatization. 



Perfusates were made by perfusing aseptically and at body 

 temperature the pancreases of cats, dogs, pigs, and oxen with 

 Locke's solution. The first perfusates were made with an alka- 

 line medium, later perfusates were made with Locke's solution 

 modified by the substitution of HC1 varying in strength from 

 0.1 per cent, to 0.7 per cent, for sodium bicarbonate. 



Respiratory quotients were obtained by a modified Jaquet 

 method, the animal being confined in a respiration chamber 

 through which a continuous stream of air was drawn for ventila- 

 tion and from which a continuous sample of air was removed for 

 analysis. In a later modification the stream of air from the 

 respiration chamber was directed into a large gasometer for a 

 twenty-minute period and the samples for analysis withdrawn 

 from this total volume. Analyses were made by the Henderson 

 and Bailey modification of Haldane's apparatus. The respiration 

 chamber was frequently checked by analyses of outside air and 

 by burning alcohol in it. 



1) :N ratios and respiratory quotients typical for diabetes were 

 obtained in the depancreatized animals. 



Following the administration of sugar in the form of dex- 

 trose or sucrose by stomach tube pancreatic perfusates were given 

 subcutaneously, intravenously, and intraperitoneally. Significant 

 increases were shown in the respiratory quotients and marked 



i dark, Admont II., Jour. Exp. Med,, 1910, xriv., 621. 

 Ibid, 1917, xxvi, 721. 



