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Scientific Proceedings (125) 



without further treatment. These proved exceedingly toxic 

 causing extensive sloughing and ulceration of the tissues into 

 which they were injected, and sometimes even caused the death 

 of the dog. These wounds were sterile unless secondarily in- 

 fected. Simple alcoholic extracts likewise produced toxic ef- 

 fects, particularly when only small volumes of alcohol were used 

 and the trypsin was for this reason not wholly destroyed. Later 

 these toxic effects were very largely eliminated hy purification 

 of the extracts. 



Tables and graphs of experimental data were given showing 

 abrupt falls in blood sugar, disappearance of urinary sugar and 

 rise of the respiratory quotient following injection of extracts. 



31 (1991) 



Three months study of the influence of the anti-diabetic substance 

 on a case of severe diabetes. 



By C. CLYDE SUTTER and JOHN R. MURL1N. 



[From the Physiological Laboratory of the University of Roches- 

 ter, Rochester, N. Y.] 



Anti-diabetic substance, prepared at the University of Roches- 

 ter was given to a case of severe diabetes mellitus (adult) by the 

 duodenal tube, by mouth and by subcutaneous injections. Ac- 

 curate study was made to determine the potency of the pancreatic 

 extract, the dosage required and the most efficient and practical 

 method of administration. 



Glycosuria had been present at least for five years and was 

 progressively increasing. Acidosis was constant during the past 

 year. At the time of beginning this study the elimination of 

 urinary sugar was greater than the intake of carbohydrate. 



The patient was placed in the Rochester General Hospital, 

 July 6, 1922, and studied while on a weighed diet consisting of 

 carbohydrate 29.5 gms., protein 69 gms., fat 107.48 gms., and to- 

 tal calories 1394. During this study the blood sugar averaged 

 from 0.410 to 0.513; the urinary sugar averaged from 32 to 45 

 grams; and the acetone and diacetic acid was 2+. 



Anti-diabetic extract was given fifteen times through the duo- 

 denal tube from July 12, 1922 to July 29, 1922 inclusive. The 

 blood sugar was reduced, reaching its lowest, 0.241 on July 26, 



