SCIENTIFIC PROCEEDINGS 



Abstracts of Communications 

 One hundred twenty-sixth meeting. 



University and Bellevue Hospital Medical College, New York City. 

 November 15, 1922. 



President Wallace in the chair. 

 33 (1993) 

 A modified Gram stain. 

 By NICHOLAS KOPELOFF and PHILIP BEERMAN. 



[From the Department of Bacteriology, Psychiatric Institute, 

 ^Yard's Island, New York City.] 



The following method of Gram staining is based upon the 

 satisfactory results obtained by the use of modifications devised 

 by Burke and Atkins : 



1. Air dry a thinly spread film and fix with least amount of 

 heat necessary to kill the organisms and fix them to the slide. 



2. Flood slide with dye solution. This is prepared by pre- 

 viously mixing in a beaker about 30 drops of a 1 per cent. 

 aqueous solution of methyl violet 6B (Coleman and Bell) with 

 8 drops of a 5 per cent, solution of sodium bicarbonate. Allow 

 the mixture to remain on slide 5 minutes or more. 



3. Flush off the excess stain with the iodine solution and 

 cover with fresh iodine solution for 2 minutes or longer. The 

 iodine solution consists of 2 gm. iodine dissolved in 10 c.c. 

 normal sodium hydroxide solution, to which is then added 90 

 c.c. of water. 



