74 



Scientific Proceedings (126) 



the acid color change and accompanying cytolysis characteristic 

 of free HC1. 



When NaHC0 3 was introduced into a stained egg the in- 

 jected area immediately turned yellow and cytolysis with liqui- 

 faction took place. The change to a yellow color and accom- 

 panying cytolysis spread throughout the cell. This showed that 

 NaOH, which can not penetrate the surface film, will exert its 

 characteristic effects if introduced directly into the interior of 

 the cell. 



The semi-permeability of a living cell is a function of its sur- 

 face film. It is immaterial whether this film be that of the 

 original cortex, a film newly formed over a cut surface, or a 

 film that surrounds an artificially induced vacuole within the cell. 

 As long as a surface film exists, neither the acid group of the 

 NH 4 C1 nor the alkaline group of the NaHC0 3 can penetrate 

 protoplasm. On the other hand, if injected beneath the surface 

 film they freely permeate the protoplasm. 



35 (1995) 



Fat transport in the body — changes in the lipoid content of the 

 blood and lymph during fat absorption in the dog. 



By H. C. ECKSTEIN (by invitation). 



[From the Sheffield Laboratory of Physiological Chemistry, 

 Yale University, New Haven, Conn.] 



After alimentary absorption of fat the content of both the 

 total fatty acids and the phosphatides of the blood is increased. 

 According to Bloor the phosphatides are synthesized from neu- 

 tral fats by the blood corpuscles. It is also conceivable that 

 phosphatide synthesis occurs during the passage of the fat com- 

 ponents through the intestinal wall. To test this hypothesis the 

 thoracic lymph and blood collected before and after introduc- 

 tion of olive oil into the duodenum of dogs previously starved 

 for 18 hours was analyzed as follows: 



